Partial Purification and Characterization of Fructosyltransferase from Aureobasidium Pullulans
نویسنده
چکیده
Fructosyltransferase enzymes was isolated and partially purified from Aureobasidium pullulans. The enzyme being intracellular was recovered from the fungal sp. by extraction method using citrate buffer (0.1 M; pH 5.5). Enzyme was Semipurified by salt fractionation method which results increase in specific activity of the enzyme. Study regarding kinetic behavior indicates that the enzyme exhibits maximal activity at pH 5.5 and at the temperature of 50°C. Mn +2 & Mg +2 are activators of enzyme while Hg 2+ is a strong inhibitor of enzyme activity. Reaction mixture resulting from enzyme action on sucrose exhibits presence of glucose, fructose and some short chain fructooligosaccharides.
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